Eco-Friendly Synthesis of Silver Nanoparticles Using Brown Sugar and Their Potential to Inhibit the Growth of Escherichia Coli

Abstract

Silver Nanoparticles (AgNPs) were synthesized in a brown sugar solution at three different temperatures (23 °C, 40 °C and 60 °C) with a concentration of 2.5 mM of AgNO₃ and 0.02 g·mL^-1 of brown sugar. The sample prepared at 60 °C exhibited the highest nanoparticle yield, with an average diameter of 22 nm, as determined by analyses of micrographs. The bactericidal effect of these nanoparticles was evaluated against Escherichia coli American Type Culture Collection (ATCC) 25922 using the microdilution method, monitoring bacterial growth via absorbance at 600 nm. After 24 hours, nanoparticles formed at 60 °C demonstrated a significant inhibitory effect on E. coli growth when used at volumes of 300 µL and 400 µL in the assay.