In Vitro Simulation of Growth and Survival of Pseudomonas aeruginosa, P. fluorescence and P. putida under Cold Shock

Abstract

The experimental demonstration of bacterial growth is of large interest within the scientific community to chalk out the specific mechanisms underlying the survival strategies under adverse conditions in nature. An array of research has been conducted on heat shock as well as the related oxidative stresses using a range of bacterial species including Escherichia coli, Bacillus spp., Salmonella spp. and Pseudomonas spp. Present study further focused on the cold shock response within the species of the ubiquitous bacterium Pseudomonas. P. aeruginosa, P. fluorescence, and P. putida were subjected to cold shock by placing the corresponding cultures at low temperatures (0 ^oC and 8 ^oC), and then their growth was assessed along with the necessary phenotypic observations. Interestingly, the pigmentation (green or bluish green pigments) was noticed almost in species at 37 ^oC compared to those growing at lower temperatures. While at 37 ^oC, P. fluorescence showed the highest growth compared to the other two strains, at 8 ^oC and even at 0 ^oC, P. putida showed the maximal growth followed by the growth of P. aeruginosa and then P. fluorescence.