Influence of Mobile Phase Composition and Temperature on the Retention Behavior of Selected Test Substances in Diol-type Column

Abstract

In this work the effect of mobile phase composition and temperature on the retention behavior of several test compounds (including non-polar and polar substances) on diol-Acclaim Mixed Mode HILIC-1 column in methanol-water systems with high (RPLC mode) and low (HILIC mode) water content was analyzed. In the studied systems both linear, slightly curved and curved van’t Hoff plots were observed. This indicates, that depending on solute physicochemical properties and mobile phase composition, both single and more complex retention mechanism(s) may influence the retention behavior of tested substances. The possibilities of different retention mechanisms existence (e.g. connected with adsorbent surface heterogeneity, hydrophobic and hydrogen bond interactions) in the analyzed systems have been also discussed.