A Novel, Simple Method for Detection of Caffeine in Urine as a Predictive Marker for Liver Diseases

Authors

  • Sarah Ali Qutb Zoology Department, Faculty of Science, Cairo University, Giza, Egypt
  • Mariam Ahmed Moustafa Zoology Department, Faculty of Science, Cairo University, Giza, Egypt
  • Mirna Fawzy Nazmi Zoology Department, Faculty of Science, Cairo University, Giza, Egypt
  • Ahmed Hany Abed Mahmoud Zoology Department, Faculty of Science, Cairo University, Giza, Egypt
  • George Safwat Mounir Abdel Masih Zoology Department, Faculty of Science, Cairo University, Giza, Egypt
  • Safa Gamal Abdel Fattah Suleiman Zoology Department, Faculty of Science, Cairo University, Giza, Egypt
  • Mary Karam Arian Amen Zoology Department, Faculty of Science, Cairo University, Giza, Egypt
  • Mohamed Omar Ewis Khamis Zoology Department, Faculty of Science, Cairo University, Giza, Egypt
  • Ayman Saber Mohamed Zoology Department, Faculty of Science, Cairo University, Giza, Egypt https://orcid.org/0000-0003-3975-4355

DOI:

https://doi.org/10.37256/acbr.2120231639

Keywords:

liver function markers, diagnosis, caffeine, urine, phosphomolybdic acid

Abstract

Drugs, chemicals, and endogenous substrates are all metabolized by hepatic cytochrome P450 (CYP) which plays a role in the aetiology of a variety of liver illnesses. Caffeine is completely metabolized by CYPs. Caffeine excretion in the urine is a sign of liver damage. The study aimed to develop and validate an inexpensive, simple, and rapid method for determining urinary caffeine as a diagnostic marker for liver diseases. Caffeine in urine sample underwent hydrolysis. Strip containing phosphomolybdic acid (PMA) was used to detect the hydrolysis product of caffeine in urine. The interaction of caffeine with PMA produced a blue color on the strip. The current method helps analyze caffeine in urine, providing a simple, rapid, and economic routine analysis.

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Published

2022-09-13

How to Cite

1.
Qutb SA, Moustafa MA, Nazmi MF, Mahmoud AHA, Masih GSMA, Suleiman SGAF, Amen MKA, Khamis MOE, Mohamed AS. A Novel, Simple Method for Detection of Caffeine in Urine as a Predictive Marker for Liver Diseases. Advanced Chemicobiology Research [Internet]. 2022 Sep. 13 [cited 2022 Dec. 1];2(1). Available from: https://ojs.wiserpub.com/index.php/ACBR/article/view/1639