A Novel, Simple Method for Detection of Caffeine in Urine as a Predictive Marker for Liver Diseases

Sarah Ali Qutb; Mariam Ahmed Moustafa; Mirna Fawzy Nazmi; Ahmed Hany Abed Mahmoud; George Safwat Mounir Abdel Masih; Safa Gamal Abdel Fattah Suleiman; Mary Karam Arian Amen; Mohamed Omar Ewis Khamis; Ayman Saber Mohamed

doi:10.37256/acbr.2120231639

A Novel, Simple Method for Detection of Caffeine in Urine as a Predictive Marker for Liver Diseases

Authors

Sarah Ali Qutb Zoology Department, Faculty of Science, Cairo University, Giza, Egypt
Mariam Ahmed Moustafa Zoology Department, Faculty of Science, Cairo University, Giza, Egypt
Mirna Fawzy Nazmi Zoology Department, Faculty of Science, Cairo University, Giza, Egypt
Ahmed Hany Abed Mahmoud Zoology Department, Faculty of Science, Cairo University, Giza, Egypt
George Safwat Mounir Abdel Masih Zoology Department, Faculty of Science, Cairo University, Giza, Egypt
Safa Gamal Abdel Fattah Suleiman Zoology Department, Faculty of Science, Cairo University, Giza, Egypt
Mary Karam Arian Amen Zoology Department, Faculty of Science, Cairo University, Giza, Egypt
Mohamed Omar Ewis Khamis Zoology Department, Faculty of Science, Cairo University, Giza, Egypt
Ayman Saber Mohamed Zoology Department, Faculty of Science, Cairo University, Giza, Egypt https://orcid.org/0000-0003-3975-4355

DOI:

https://doi.org/10.37256/acbr.2120231639

Keywords:

liver function markers, diagnosis, caffeine, urine, phosphomolybdic acid

Abstract

Drugs, chemicals, and endogenous substrates are all metabolized by hepatic cytochrome P450 (CYP) which plays a role in the aetiology of a variety of liver illnesses. Caffeine is completely metabolized by CYPs. Caffeine excretion in the urine is a sign of liver damage. The study aimed to develop and validate an inexpensive, simple, and rapid method for determining urinary caffeine as a diagnostic marker for liver diseases. Caffeine in urine sample underwent hydrolysis. Strip containing phosphomolybdic acid (PMA) was used to detect the hydrolysis product of caffeine in urine. The interaction of caffeine with PMA produced a blue color on the strip. The current method helps analyze caffeine in urine, providing a simple, rapid, and economic routine analysis.

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Published

2022-09-13

How to Cite

Qutb SA, Moustafa MA, Nazmi MF, Mahmoud AHA, Masih GSMA, Suleiman SGAF, Amen MKA, Khamis MOE, Mohamed AS. A Novel, Simple Method for Detection of Caffeine in Urine as a Predictive Marker for Liver Diseases. Advanced Chemicobiology Research [Internet]. 2022 Sep. 13 [cited 2024 Apr. 26];2(1):23-9. Available from: https://ojs.wiserpub.com/index.php/ACBR/article/view/1639

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Issue

Advanced Chemicobiology Research, Volume 2 Issue 1 (2023)

Section

Research Article

License

Copyright (c) 2022 Sarah Ali Qutb, Mariam Ahmed Moustafa, Mirna Fawzy Nazmi, Ahmed Hany Abed Mahmoud, George Safwat Mounir Abdel Masih, Safa Gamal Abdel Fattah Suleiman, Mary Karam Arian Amen, Mohamed Omar Ewis Khamis, Ayman Saber Mohamed

This work is licensed under a Creative Commons Attribution 4.0 International License.