Inhibitory Activity of Red and Yellow Araçá Genotypes Towards Carbohydrate-Hydrolyzing Enzymes: Putative Role of Ellagitannins

Authors

  • Juliana Vinholes Embrapa Temperate Climate, BR 392, CEP 96010-971, Pelotas-RS, Brazil
  • Graciele Lemos Embrapa Temperate Climate, BR 392, CEP 96010-971, Pelotas-RS, Brazil
  • Juliana Hofstätter Azambuja Department of Basic Health Sciences, Federal University of Health Sciences of Porto Alegre, Rua Sarmento Leite, 245, CEP 90.050-170, Porto Alegre-RS, Brazil
  • Sofia Filipa Reis Network of Chemistry and Technology/Associated Laboratory for Green Chemistry (REQUIMTE/LAQV), Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Portugal
  • Alisa Rudnitskaya Centre of Environmental and Marine Studies (CESAM), Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal
  • Elizandra Braganhol Department of Basic Health Sciences, Federal University of Health Sciences of Porto Alegre, Rua Sarmento Leite, 245, CEP 90.050-170, Porto Alegre-RS, Brazil
  • Victor de Freitas Network of Chemistry and Technology/Associated Laboratory for Green Chemistry (REQUIMTE/LAQV), Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, Rua do Campo Alegre, 687, 4169-007, Porto, Portugal
  • Rodrigo Cezar Franzon Embrapa Temperate Climate, BR 392, CEP 96010-971, Pelotas-RS, Brazil
  • Ana Reis Network of Chemistry and Technology/Associated Laboratory for Green Chemistry (REQUIMTE/LAQV), Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, Rua do Campo Alegre, 687, 4169-007, Porto, Portugal https://orcid.org/0000-0002-4537-9902
  • Márcia Vizzotto Embrapa Temperate Climate, BR 392, CEP 96010-971, Pelotas-RS, Brazil

DOI:

https://doi.org/10.37256/fse.5220244850

Keywords:

native fruits, diabetes, phytochemicals, α-amylase, α-glucosidase, sucrase

Abstract

Psidium cattleianum Sabine (araçá) is a species native to Southeast Brazil that grows under abiotic stress conditions conferring high content of bioactive compounds to its fruits. The presence of these compounds is thought to be responsible for the many health-promoting effects including antioxidant, anti-inflammatory, anti-aging and antidiabetic activities. In this study, we evaluated the inhibitory potential of 10 (red and yellow) araçá genotypes towards carbohydrate-hydrolyzing enzymes (CHEs) using cell-free (α-glucosidase, α-amylase) and cell-based assays (sucrase). Araçá extracts displayed stronger inhibition towards α-glucosidase than α-amylase, and only 3 inhibited sucrase activity. The high variability towards the in vitro inhibitory CHEs activity was reflected in the total phenolics content with values ranging between 38.9 and 117 mg/100 g. Of the thirty compounds identified by High-Performance Liquid Chromatography-Diode Array Detection-Electrospray Ionization-Tandem Mass Spectrometry (HPLC-DAD-ESIMS/MS), including caffeic acids (9), organic acids (3) ellagitannins (15) and flavonoids (3), ellagitannins were the most abundant class. Statistical analysis showed ellagitannins were the main discriminators to the CHEs inhibitory activity. In summary, by expanding the panel of red and yellow araçá varieties studied, our results show that not all araçá genotypes inhibit CHE as only YA-23, RA-29, and RA-87 inhibited all 3 CHE which were related to the presence of ellagitannins. Information on the araçá genotypes with greater CHE inhibitory activity allied with the health-promoting effects of ellagitannin-rich foods, can be used to scale-up commercially exploitable genotypes with the aim to develop araçá-containing food formulations targeted to the pre-diabetic population.

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Published

2024-08-13

How to Cite

1.
Vinholes J, Lemos G, Azambuja JH, Reis SF, Rudnitskaya A, Braganhol E, Freitas V de, Franzon RC, Reis A, Vizzotto M. Inhibitory Activity of Red and Yellow Araçá Genotypes Towards Carbohydrate-Hydrolyzing Enzymes: Putative Role of Ellagitannins. Food Science and Engineering [Internet]. 2024 Aug. 13 [cited 2024 Oct. 11];5(2):300-21. Available from: https://ojs.wiserpub.com/index.php/FSE/article/view/4850